Interactive Effects of Indole-3-butyric Acid, Arbuscular Mycorrhizal Fungi, and Rooting Media on Rooting of Vitis vinifera L. cv. Sistan Yaghooti Cuttings

Bidarnamani, Fatemeh; Mohkami, Zeinab; Elahi-Moghaddam, Ehsan

doi:10.22034/jgpp.2026.742909

Interactive Effects of Indole-3-butyric Acid, Arbuscular Mycorrhizal Fungi, and Rooting Media on Rooting of Vitis vinifera L. cv. Sistan Yaghooti Cuttings

Document Type : Original Article

Authors

Fatemeh Bidarnamani ¹

Zeinab Mohkami ¹

Ehsan Elahi-Moghaddam ²

¹ Agriculture Institute, Research Institute of Zabol, Zabol, Iran

² Master of Natural Resources, Fisheries, Agriculture Institute, Research Institute of Zabol, Zabol, Iran

https://doi.org/10.22034/jgpp.2026.742909

Abstract

Efficient vegetative propagation of grapevine depends on successful adventitious root formation, which is influenced by genotype, auxin supply, rooting environment, and biological interactions. The present study evaluated the individual and interactive effects of rooting medium, indole-3-butyric acid (IBA), and arbuscular mycorrhizal fungi (AMF) on the rooting performance of Vitis vinifera L. cv. Sistan Yaghooti cuttings. A three-factor factorial experiment was conducted under a completely randomized design using three rooting media (sand, soil, and cocopeat + perlite), three IBA concentrations (0, 2000, and 4000 mg L⁻¹), and four fungal treatments (non-inoculated control, Glomus intraradices, G. mossaea, and SuperMychoPlus). Rooting success and root-system traits were evaluated after approximately two months. Analysis of variance revealed significant effects and interactions among rooting medium, IBA concentration, and fungal treatment for several rooting traits, including significant three-way interactions. These results indicate that rooting responses strongly depended on the specific combination of IBA concentration, fungal treatment, and rooting medium. For example, the sand + 2000 mg L⁻¹ IBA combination averaged 2.42 rooted cuttings per experimental unit, corresponding to 40.3% rooting, whereas the soil + untreated control averaged 0.58 rooted cuttings (9.7%). Several cocopeat + perlite combinations supported greater development of root-system traits, including root number and total root length. Thus, rooting success and root-system development did not necessarily follow the same response pattern. Overall, the findings indicate that propagation of Sistan Yaghooti grapevine should be evaluated using integrated treatment combinations rather than considering IBA concentration, fungal treatment, or rooting medium independently.

Keywords

Adventitious Roots

Cocopeat

Glomus Intraradices

Glomus Mosseae

Vegetative Propagation

Yaghooti

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