Effects of foliar and fertigation based calcium nitrate applications on growth, flower quality, and postharvest physiology of roses in a soilless system

Document Type : Original Article

Authors

1 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Department of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

3 Ferdowsi University of Mashhad. Faculty of Agriculture.

Abstract
This study investigated the effects of foliar and fertigation-based calcium nitrate applications on growth, flower quality, and postharvest physiology of two Rosa hybrida cultivars (‘Samurai’ and ‘Jumilia’) grown in a soilless system. Treatments included a control (10% of the recommended Ca level), foliar spraying, and fertigation, arranged in a factorial design with four replications. The experiment was conducted in a greenhouse at the Faculty of Agriculture, Ferdowsi University, Mashhad, Iran. Results revealed that root morphological characteristics such as length and volume were maximized in the ‘Jumilia’ cultivar when calcium nitrate was supplied via fertigation. Conversely, foliar spraying with calcium nitrate markedly improved shoot and floral characteristics. Compared to the control, foliar calcium nitrate increased flower diameter by 15.4%, enhanced hip length by 14.25 cm, and increased hip diameter in the ‘Samurai’ cultivar by 18.76%. Moreover, foliar application of calcium nitrate extended the vase life of cut roses to about 10 days by preserving higher relative fresh weight. Antioxidant enzyme activities were also enhanced under foliar treatment in the ‘Samurai’ cultivar, with superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) increasing by 53.84%, 75.71%, and 97.28%, respectively. Additionally, foliar-sprayed ‘Samurai’ plants showed nearly double calcium accumulation in leaves and petals. Interestingly, in the ‘Jumilia’ cultivar, foliar application reduced leaf zinc content by 68.87%, while simultaneously enhancing leaf magnesium concentration by 64.16%. Overall, foliar supplementation with calcium nitrate proved to be a practical strategy for improving flower quality, enhancing antioxidant defense, and prolonging vase life of roses in soilless culture.

Keywords


  1. Abdolmaleki, M., Khosh-Khui, M., Eshghi, S., and Ramezanian, A. 2015. Improvement in vase life of cut rose cv.“Dolce Vita” by preharvest foliar application of calcium chloride and salicylic acid. International Journal of Horticultural Science and Technology, 2, 55-66.
  2. Aghdam, M., Asil, M.H., Ghasemnezhad, M., and Mirkalaei, S.M. 2019. Effects of pre-harvest applications of different source of calcium on the cell wall fractions and stem bending disorder of Gerbera (Gerbera jamesonii L.) cultivar flowers. Advances in Horticultural Science, 33,57-66.
  3. Ahmad, M., and Rab, A. 2020. Calcium effects on post-harvest attributes and vase life of gladiolus using different methods of application. Pakistan Journal of Botany, 52, 167-179. https://doi.org/10.30848/PJB2020-1(28)
  4. Albino-Garduño, R., Zavaleta-Mancera, H. A., Ruiz-Posadas, L. M., Sandoval-Villa, M., and Castillo-Morales, A. 2007. Response of gerbera to calcium in hydroponics. Journal of Plant Nutrition, 31, 91-101. https://doi.org/10.1080/01904160701741958
  5. Al-Ibraheemi, R.A., Alrubye, H.K., Mashkoor, S.A., and Alaunaibi, R.M. 2021. The effect of calcium on the growth and flowering of the rose moss Portulaca grandiflora L. using hydroponic system. Nat. NVEO-Natural Volatiles & Essential Oils Journal| NVEO, 2531-2535.
  6. Alikhani, T.T., Tabatabaei, S.J., Torkashvand, A.M., and Talei, D. 2021. Silica nanoparticles and calcium on the histological characteristics and stem bending in gerbera cut flower. Ornamental Horticulture, 27, 334-343. https://doi.org/10.1590/2447-536x.v27i3.2308
  7. AsgariI Gouraj,M. 2025. Gaseous enrichment of vase solution enhances vase life and antioxidant capacity in Matthiola incana cv. ‘Lucinda’. Greenhouse Plant Production Journal, 2(3), 62-73. https://doi: 10.61882/gppj.2.3.62
  8. Azeez, D.R., Medan, R.A., and Hussein, S.A. 2017. Effect of foliar application of chelated calcium and boron on growth and yield of strawberry (Fragaria ananassa Duch.) CV. Festival. Kirkuk University Journal For Agricultural Sciences, 8.
  9. Banijamali, S.M., Feizian, M., Bayat, H., and Mirzaei, S. 2018. Effects of nitrogen forms and calcium amounts on growth and elemental concentration in Rosa hybrid cv. ‘Vendentta’. Journal of Plant Nutrition, 41, 1205-1213. https://doi.org/10.1080/01904167.2018.1443127
  10. Chanasut, U., Rogers, H.J., Leverentz, M.K., Griffiths, G., Thomas, B., Wagstaff, C., and Stead, A.D. 2003. Increasing flower longevity in Alstroemeria. Postharvest Biology and Technology, 29, 325-333. https://doi.org/10.1016/S0925-5214(03)00048-6
  11. Chen, C., Lu, S., Chen, Y., Wang, Z., Niu, Y., and Guo, Z. 2009. A gamma-ray–induced dwarf mutant from seeded bermudagrass and its physiological responses to drought stress. The American Society for Horticultural Science, 134, 22-30. https://doi.org/10.21273/JASHS.134.1.22
  12. Combrink, N.J. 2018. Calcium improves gerbera (Gerbera hybrida) vase life. South African Journal of Plant and Soil, 35,235-236. https://doi.org/10.1080/02571862.2017.1354089
  13. Davarzani, M., Aliniaeifard, S., Zare Mehrjerdi, M., and Roozban, M. R. 2025. Enhancing the vase life of cut roses through spectral optimisation during greenhouse cultivation. Annals of Applied Biology. https://doi.org/10.1111/aab.70083
  14. Dehestani,M. M. , Heidari,M. , Sabbagh,S. K. , Nadaf,M. and Baradaran Firouzabadi,M. 2025. Evaluate effect of mycorrhizal, zinc and iron oxide on root growth, antioxidant enzyme activities, and mineral elements in sorghum (Sorghum bicolor). Greenhouse Plant Production Journal, 2(3), 33-39. https://doi: 10.61882/gppj.2.3.33
  15. Desta, B., Tena, N., and Amari. G. 2022. Response of rose (Rosa hybrida L.) plant to temperature. Asian Journal of Plant and Soil Sciences,7, 93-101. https://doi.org/10.56557/ajopss/2022/v7i179
  16. Ehsanimehr, N., Hosseinifarahi, M., Abdipour, M., Eshghi, S., and Jamali, B. 2024. Improving postharvest quality and vase life of cut rose flowers by pre-harvest foliar co-applications of γ-Aminobutyric Acid and calcium chloride through antioxidant contents. Research Square, 1-22. https://doi.org/10.1038/s41598-024-64021-8
  17. Farazmandi, M., Mirzakhani, A., Sajedi, N.A., Nasri, M. and Gomariyan, M. 2020. Physiological characteristics and vase life responses of rose cut flowers (Rosa hybrida L. cv.‘Royal Baccara’) to benzyl adenine and 1-metylcylcopropene. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48. https://doi.org/10.15835/nbha48411790
  18. Faust, M., and Klein, J.D. 1974. Levels and sites of metabolically active calcium in apple fruit. Journal of the American Society for Horticultural Science,99,93–94. https://doi.org/10.21273/JASHS.99.1.93
  19. Ferguson, I.B., and Drøbak, B.K. 1988. Calcium and the regulation of plant growth and senescence. Horticultural Science, 23, 262-266. https://doi.org/10.21273/HORTSCI.23.2.262a
  20. Ghadimian, S., Danaei, E., 2020. Influences of ascorbic acid and salicylic acid on vase life of cut flowers rose (Rosa hybrida cv. black magic). Alkhas; The Journal of Environment, Agriculture and Biological Sciences, 2, 1-6. https://doi.org/10.29252/alkhass.2.1.1
  21. Ha, S. T. T., In, B. C., and Lim, J. H. 2024. LED light improves postharvest quality and longevity of cut rose flowers 'Lovely Lydia'. Acta Horticulturae, 1291, 261–268.
  22. Hashemabadi, D., Kaviani, B., Nekouyar, N., Ezzatpazhoh, S., and Zaredoost, F. 2021. Effect of warm water on postharvest quality of cut rose (Rosa hybrida L.) flowers. Ornamental Horticulture, 27, 351-356. https://doi.org/10.1590/2447-536x.v27i3.2336
  23. Hong, L.T.M., Trinh,T.C., Bui, V.T., and Tran, H.T. 2021. Roles of plant growth regulators on flowering of rose (Rosa hybrida L.’Red Rose’). In IOP Conference Series: Environmental Earth Sciences ,947, 012039. https://doi.org/10.1088/1755-1315/947/1/012039
  24. Javad, N.D.M., Ahmad, K., Mostafa, A., and Roya, K. 2011. Postharvest evaluation of vase life, stem bending and screening of cultivars of cut gerbera (Gerbera jamesonii Bolus ex. Hook f.) flowers. African Journal of. Biotechnology, 10, 560-566.
  25. Jiang, Y., and Huang, B. 2001. Effects of calcium on antioxidant activities and water relations associated with heat tolerance in two coolseason grasses. Journal of Experimental Botany, 355,341-349. https://doi.org/10.1093/jexbot/52.355.341
  26. Johnson, J.M., Ludwig, A., Furch, A.C., Mithöfer, A., Scholz, S., Reichelt, M., and Oelmüller, R. 2019. The beneficial root-colonizing fungus Mortierella hyalina promotes the aerial growth of Arabidopsis and activates calcium-dependent responses that restrict Alternaria brassicae–induced disease development in roots. Molecular Plant-Microbe Interactions, 32, 351-363. https://doi.org/10.1094/MPMI-05-18-0115-R
  27. Kazemi,S. , Pirmoradi,M. R. , Raghami,M. and Malekzadeh,M. R. 2024. Enhancing the absorption of microelements by applying humic acid and zinc sulfate in Physalis alkekengi: Improve chlorophyll content and fruit quality. Greenhouse Plant Production Journal, 1(3), 68-82. https://doi: 10.61186/gppj.1.3.68
  28. Khalaj, M.A., and Noroozisharaf, A. 2020. Efficiency of ammonium and nitrate ratios on macronutrient content and morphological properties of Gerbera jamesonii cut flower. Agriculturae Conspectus Scientificus,85, 281-289.
  29. Khalaj, M.A., Azimi, M.H., Edrisi, B., and Sayyad-Amin, P. 2023. Growth, flowering, and durability of Gladiolus affected by foliar application of calcium from different sources and at different doses. Journal of Ornamental Plant,  13,53-65.
  30. Khalaj, M.A., Kiani, S., Khoshgoftarmanesh, A.H., and Amoaghaie, R. 2017. Growth, quality, and physiological characteristics of gerbera (Gerbera jamesonii L.) cut flowers in response to different NO3−: NH4+ ratios. Horticulture, Environment, and Biotechnology, 58,13-323. https://doi.org/10.1007/s13580-017-0067-7
  31. Li, Z., Gemma, H., and Iwahori, S. 2002. Stimulation of ‘Fuji’apple skin color by ethephon and phosphorus–calcium mixed compounds in relation to flavonoid synthesis. Scientia Horticulturae, 94,193-199. https://doi.org/10.1016/S0304-4238(01)00363-6
  32. Liu, W., Muzolf-Panek, M., and Kleiber, T. 2023. Effect of Varied Nitrogen Sources and Type of Cultivation on the Yield and Physicochemical Parameters of Flowering Chinese Cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee). Applied Sciences,13, 5691. https://doi.org/10.3390/app13095691
  33. Mahajan, M., and Pal, P.K. 2020. Flower yield and chemical composition of essential oil from Rosa damascena under foliar application of Ca (NO3) 2 and seasonal variation. Acta Physiologiae Plantarum, 42,23. https://doi.org/10.1007/s11738-019-2996-5
  34. Marschner, H. 2012. Mineral nutrition of higher plants., Academic, Australia
  35. Milioni, C.B., Kawakubo, L.H., Kanashiro, R.J., Santos, V.R.D., Kanashiro, S., and Tavares, A.R. 2019. Effect of calcium fertilization on silver vase bromeliad (Aechmea fasciata). Ornamental Horticulturae, 25, 126-132. https://doi.org/10.14295/oh.v25i2.1230
  36. Mohammadbagheri, L., and Naderi, D. 2017. Effect of growth medium and calcium nano-fertilizer on quality and some characteristics of gerbera cut flower. Journal of Ornamental Plant, 7,205-213.
  37. Mohammed, R.A.J., and Abood, B.M.A. 2020. Effect of bacterial inoculum, spraying with calcium nitrate and salicylic acid in vegetative and flowery growth traits of Gerbera jamesonii. Plant Archives. 20, 633-638.
  38. Moradinezhad, F., S. Hassanpour and M. H. Sayyari, 2018. Influence of Pre harvest Spray of Calcium Chloride and Salicylic Acid on Physicochemical and Quality Properties of Fresh Seedless Barberry Fruit. Journal of Horticultural Science. 32: 61-74.
  39. Mortazavi, N., Naderi, R., Khalighi, A., Babalar, M., and Allizadeh, H. 2007. The effect of cytokinin and calcium on cut flower quality in rose (Rosa hybrida L.) cv. Illona. Journal of Food, Agriculture and Environment, 5, 311-313.
  40. Ramasubramanian, S., Jegadeeswari, V., Vijayalatha, K. R., Kaleeswari, R. K., Nithila, S., Iyyamperumal, M., and Jayaraman, K. 2025. Effect of fertigation on soil nutrients, microbial activity, plant and fruit(s) growth parameters grown under tropical environments: A discussion. Communications in Soil Science and Plant Analysis, 56(17), 1–29. https://doi.org/10.1080/00103624.2025.2522190
  41. Robichaux, M.B.L. 2008. The effect of calcium or silicon on potted miniature roses or poinsettias. Louisiana State University Agricultural Mechanical College. 1-96
  42. Roosta, H., Nili, F., Pourkhaloee, A., and Askar, N. 2024. Effects of Supplemental Light Quality and Foliar Application with Calcium on Photosynthetic Parameters and Flower Stem Strength of Cut Gerbera (Gerbera jamesonii ‘Bayadere’). International Journal of Horticultural Science and Technology ,11(1), 69-82.
  43. Saikia, P., Talukdar, M.C., and Das, P. 2018. Optimization of zinc, magnesium and calcium on growth and flowering of Rhynchostylis Retusa L. Acta Scientific Agriculture, 4, 12-15.
  44. Sairam, R. K., Vasanthan, B., and Arora, A. 2011. Calcium regulates Gladiolus flower senescence by influencing antioxidative enzymes activity. Acta Physiologiae Plantarum,33, 1897-1904.
  45. Seyedi, N., MohammadiTorkashvand, and A., Allahyari, M.S. 2015. Investigating of the effects of calcium concentration under hydroponic conditions on quantitative and qualitative growth of Lilium ‘tresor. Journal of Ornamental Plant, 3, 19-24.
  46. Shamsinejad,S. , Saffari,V. R. , Pakkish,Z. and Saadati,S. 2024. Combination of Different Concentrations and Application Methods of Melatonin: Controls postharvest spoilage, maintains quality, and extends shelf-life of Cut Rose Flowers. Greenhouse Plant Production Journal, 1(4), 93-112. https://doi: 10.61186/gppj.1.4.93
  47. Sharifi, B., and Naderi, D. 2019. Effects of Some Mineral Substrates on Qualitative and Quantitative Traits of Tuberose (Plianthes tuberosa L.). International Journal of Horticultural Science and Technology, 6,101-112.
  48. Sobczak, A., Pióro-Jabrucka, E., Gajc-Wolska, J., and Kowalczyk, K. 2024. Effect of salicylic acid and calcium on growth, yield, and fruit quality of pepper (Capsicum annuum L.) grown hydroponically. Agronomy, 14,329. https://doi.org/10.3390/agronomy14020329
  49. Soufi,H. R. , Shojaee Khabisie,M. , Ramezan,D. , Zeinali Pour,N. and Zakeri marvast,H. 2025. Effects of Different Nitrogen Sources and Sodium Bicarbonate on Growth and Nutrient Uptake in Two Garlic Genotypes: A Hydroponic Study. Greenhouse Plant Production Journal, 2(1), 44-72. https://doi: 10.61186/gppj.2.1.44
  50. Torre, S., Borochov, A., and Halevy, A.H. 1999.Calcium regulation of senescence in rose petals. Physiologia Plantarum, 107, 214-219 https://doi.org/10.1034/j.1399-3054.1999.100209.x
  51. Volpin, H., and Elad, Y. 1991. Influence of calcium nutrition on susceptibility of rose flowers to botrytis blight. Journal of Physiology and Biochemistry, 81,1390– 1394. https://doi.org/10.1094/Phyto-81-1390
  52. Wagner, G.J. 1979. Content and vacuole extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology, 64, 88–93. https://doi.org/10.1104/pp.64.1.88
  53. Watane, A.A., Dalal, S.R., Gaidhani, A., and Gupta, N.S. 2022. Studies on influence of plant density and calcium nitrate spray on growth, flowering and yield of China aster. Journal of Pharmaceutical Innovation..11, 2300-2304.
  54. White, P.J., and Broadley, M.R. 2003. Calcium in plants. Annals of Botany, 92, 487-511. https://doi.org/10.1093/aob/mcg164
  55. Zhao, D., Tang, Y., Xia, X., Sun, J., Meng, J., Shang, J., and Tao, J. 2019. Integration of transcriptome, proteome, and metabolome provides insights into how calcium enhances the mechanical strength of herbaceous peony inflorescence stems. Cells, 8, 102. https://doi.org/10.3390/cells8020102
Volume 2, Issue 4
Autumn 2025
Pages 65-80

  • Receive Date 10 October 2025
  • Revise Date 15 November 2025
  • Accept Date 10 December 2025
  • First Publish Date 10 December 2025
  • Publish Date 01 December 2025