Greenhouse Plant Production Journal
Quarterly

Combination of Different Concentrations and Application Methods of Melatonin: Controls postharvest spoilage, maintains quality, and extends shelf-life of Cut Rose Flowers

Document Type : Original Article

Authors

Sara Shamsinejad 1
Vahid Reza Saffari 1
Zahra Pakkish 2
Safoora Saadati 1

1 Department of Horticultural Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran

2 Department of Horticultural Science, Faculty of Agriculture, ShahidBahonar University of Kerman, Iran

https://doi.org/10.61186/gppj.1.4.93
Abstract
To date, various preservative solutions and chemicals have been introduced to extend the postharvest longevity of cut flowers, and research in this field holds significant economic and horticultural importance. In this study, the response of cut rose flowers to different application methods and concentrations of melatonin was investigated. The experiment was carried out based on a completely randomized design, with two treatment methods: short-term (pulse) and continuous application, using different concentrations and five replications per treatment. In the short-term (pulse) method, the samples were placed in a melatonin solution for a specified duration after re-cutting, and then each sample was transferred to a vase solution. The pulse treatments included melatonin concentrations of 0.1, 0.5, 1, and 2 mM, each applied for 30 minutes. Additionally, the 0.5 mM melatonin concentration was applied for 2 and 4 hours. Continuous treatments were applied at three concentrations (0.01, 0.1, and 1 mM) until the end of the vase life. The results indicated that melatonin treatments had a significant effect on vase life compared to the control. The longest vase life was observed with the 0.1 mM pulse treatment. It appears that melatonin application helps reduce ion leakage. Exhibited superior retention of water uptake, relative moisture content, chlorophyll, and carotenoid levels. The greater effectiveness of the pulse treatment compared to the continuous treatment may be attributed to the sensitivity and instability of melatonin.

Keywords

Ion leakage
Longevity
Melatonin
Oxidative stress
Treatment
  1. Aba-Alkhail, A.A. 2005. Antifungal activity of some extracts against some plant pathogenic fungal. Pakistan Journal of Biological Sciences, 8(3): 413-417.
  2. Abbasi, N. A., Hafeez, S. and Tareen, M. J. 2010. Salicylic acid prolongs shelf life and improves quality of "Mari Delicia" peach fruit. ActaHorticulturae, 880: 191-197.
  3. Abd-El-Khair, H., Omima, M.H. 2006. Effect of aqueous extracts of some medicinal plant in controlling the Green mould disease and improvement of stored "Washington" navel orange quality. Journal of Applied Sciences Research, 2(10): 664-674.
  4. Aalifar, M.; Aliniaeifard, S.; Arab, M.; Mehrjerdi, M.Z.; Serek, M. 2020. Blue light postpones senescence of carnation flowers through regulation of ethylene and abscisic acid pathway-related genes. Plant Physiology and Biochemistry, 151: 103-112.
  5. Aghdam, M.S.; Luo, Z.; Li, L.; Jannatizadeh, A.; Fard, J.R.; Pirzad, F. 2020. Melatonin treatment maintains nutra-ceutical properties of pomegranate fruits during cold storage. Food Chemistry, 303: 3-55.
  6. Akhtar, G.; Rajwana, I.A.; Sajjad, Y.; Asif, M.; Muhammad, S.; Kashif, A.; Sami, R.; Hafiz, U.; Faried, N.; Farooq, A. 2021. Do natural leaf extracts involve regulation at physiological and biochemical levels to extend vase life of gladiolus cut flowers. ScientiaHorticulturae, 282: 110047.
  7. Anderson, N.O. 2016. Flower breeding and genetics, Issues, challenges and opportunities for the 21 century. Netherlands. 700 pp.
  8. Arnao, M.B., Hernández-Ruiz, J. 2017. Melatonin and its relationship to plant hormones. Annals of Botany, 121(2): 195-207.
  9. Arteca, R.N. 1996. Plant Growth Substances: Principle and applications. New York: Chapman & Hall.
  10. Asaoka, M., Heins, R.D. 1982. Influence of supplemental light and preforcing storage treatments on the forcing of 'Red Garnet' rose as a pot plant. HortScience, 107: 548-552.
  11. Ban, Z., Zhang, S., Niu, C., Liu, L., Cao, K., Li, L., Wu, Z., Wang, L., Chen, C., & Zhu, Y. (2023). Potential role of exogenous melatonin involved in postharvest quality maintenance of Vitis labrusca × Vitis vinifera ‘Kyoho’. Journal of the Science of Food and Agriculture, 103(15), 6243–6251.
  12. Benner, J.P. 1993. Pesticidal compounds from higher plants. Pesticide Science, 39: 95-102.
  13. Bleeksma, H.C., Van Doorn, W.G. 2003. Embolism in rose stems as a result of vascular occlusion by bacteria. Postharvest Biology and Technology, 29: 334-340.
  14. Cairns, T. 2005. Modern roses XI, the world encyclopedia of roses. San Diego: Academic Press.
  15. Carrion-Antoli, A., Martinez-Romero, D., & Guillen, F. (2022). Melatonin pre-harvest treatments leads to maintenance of sweet cherry quality during storage by increasing antioxidant systems. Frontiers in Plant Science, 13, 863467.
  16. Chanasut, U., Rogers, H.J., Leverentz, M.K., Griffiths, G., Thomas, B., Wagstaff, C., Stead, A.D. 2003. Increasing flower longevity in Alstroemeria. Postharvest Biology and Technology, 29: 324-332.
  17. Chang, H., Cheng, Y., Wu, C., Chang, S., Chang, T., Su, Y. 2008. Antifungal activity of essential oils and its constituents from Calocedrusmacrolepis var. formosana Florin leaf against plant pathogenic fungi. Bioresource Technology, 99: 6266-6270.
  18. Damunupola, J.W., Qian, T., Muusers, R., Joyce, D.C., Irving, D.E., Van Meeteren, U. 2010. Effect of S-carvone on vase life parameters of selected cut flower and foliage species. Postharvest Biology and Technology, 55: 66-69.
  19. Debnath, B.; Islam, W.; Li, M.; Sun, Y.T.; Lu, X.C.; Mitra, S.; Hussain, M.; Liu, S.; Qiu, D. 2019. Melatonin mediates enhancement of stress tolerance in plants. International Journal of Molecular Sciences, 20(5): 1040.
  20. Dole, J.M., Wilkins, H.F. 2009. Floriculture: Principles and Species. New Jersey: Prentice-Hall. 613p.
  21. Eason, J.R., Ryan, D.J., Pinkney, T.T., O'Donoghue, E.M. 2002. Programmed cell death during flower senescence: isolation and characterization of cysteine proteinases from Sandersoniaaurantiaca. Functional Plant Biology, 29: 1055-1064.
  22. Elkovich, S.D. 2017. Terpenoids from the genus Artemisia as potential pesticides. In: Cutler, H.G. (ed.), Natural products and their potential role in agriculture: ACS symposium series 380. Washington DC: American Chemical Society, pp. 250-261.
  23. Fan, J.; Xie, Y.; Zhang, Z.; Chen, L. 2018. Melatonin: A multifunctional factor in plants. International Journal of Molecular Sciences, 19(5): 1528.
  24. Faragher, D.J. 1985. Effects of Inhibitor of Ethylene Action, Silver Thiosulfate, on Vase Life and Development of Fresh and Cold Stored Flowers. Ph.D thesis, Hebrew University of Jerusalem, Australia.
  25. Genena, A.K, Hense, H., Smania Junior, A., Machado de Souza, S. 2008. Rosemary (Rosmarinusofficinalis) a study of the composition, antioxidant and antimicrobial activities of extracts obtained with supercritical carbon dioxide. Ciência e Tecnologia de Alimentos, 28(2): 463-469.
  26. Gulluce, M., Sokmen, M., Daferera, D., Agar, G., Ozkan, H., Kartal, N., Polissiou, M., Sokmen, A., Sahin, F. 2003. In-vitro antibacterial, antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of SaturejahortensisL. Journal of Agricultural and Food Chemistry, 51(14): 3958-3965.
  27. Hadizadeh, I., Peivastegan, B., Hamzehzarghani, H. 2009. Antifungal activity of essential oils from some medicinal plants of Iran against Alternariaalternata. American Journal of Applied Sciences, 6(5): 744-748.
  28. Halevy, A.H., Mayak, S. 2007. Senescence and postharvest physiology of cut flowers. Part 2. In: Janick, J. (ed.), Horticultural Reviews 2. Westport, CT: AVI Publishing.
  29. Hassan, F.A.S.; Mazrou, R.; Gaber, A.; Hassan, M. 2020. Moringa extract preserved the vase life of cut roses through maintaining water relations and enhancing antioxidant machinery. Postharvest Biology and Technology, 164: 111156.
  30. Hatamzadeh, A.; Hatami, M.; Ghasemnezhad, M. 2012. Efficiency of salicylic acid delay petal senescence and extended quality of cut spikes of Gladiolus grandiflora cv. Wing's Sensation'. African Journal of Agricultural Research, 7(4): 540-545.
  31. Hei, H., Tang, H., Zhao, R., Li, G., & Shi, F. (2025). Effect of melatonin treatment on storage quality and antioxidant system of postharvest winter jujube (Zizyphus jujuba Mill. cv. Dongzao). Foods, 14(3), 576.
  32. Hernández-Ruiz, J.; Arnao, M.B. 2018. Relationship of melatonin and salicylic acid in biotic/abiotic plant stress responses. Agronomy, 8(4): 33.
  33. Hessini, K.; Wasli, H.; Al-Yasi, H.M.; Ali, E.F.; Issa, A.A.; Hassan, F.A.S.; Siddique, K.H.M. 2022. Graded moisture deficit effect on secondary metabolites, antioxidant, and inhibitory enzyme activities in leaf extracts of Rosa damascena Mill. var. trigentipetala. Horticulturae, 8(3): 177.
  34. Lezoul, N.E.; Serrano, M.; Ruiz-Aracil, M.C.; Belkadi, M.; Castillo, S.; Valero, D.; Guillén, F. 2022. Melatonin as a new postharvest treatment for increasing cut carnation (Dianthus caryophyllus L.) vase life. Postharvest Biology and Technology, 184: 111177.
  35. Li, P., Zhao, R., Zhou, H., Wu, S., & Zhang, X. (2024). Melatonin delays softening of postharvest pepper fruits (Capsicum annuum L.) by regulating cell wall degradation, membrane stability and antioxidant systems. Postharvest Biology and Technology, 212, 112852.
  36. Li, Z., Zhang, S., Xue, J., Mu, B., Song, H., & Liu, Y. (2022). Exogenous melatonin treatment induces disease resistance against Botrytis cinerea on post-harvest grapes by activating defence responses. Foods, 11(14), 2231.
  37. Lu, P.; Cao, J.; He, S.; Liu, J.; Li, H.; Cheng, G.; Ding, Y.; Joyce, D.C. 2010. Nano-silver pulse treatments improve water relations of cut rose cv. Movie Star flowers. Postharvest Biology and Technology, 57(3): 196-202.
  38. Ma, Q., Lin, X., Wei, Q., Yang, X., Zhang, Y., & Chen, J. (2021). Melatonin treatment delays postharvest senescence and maintains the organoleptic quality of ‘Newhall’ navel orange (Citrus sinensis (L.) Osbeck) by inhibiting respiration and enhancing antioxidant capacity. Scientia Horticulturae, 286, 110236.
  39. Mazrou, R.M., Hassan, S., Yang, M. and Hassan, F.A. 2022. Melatonin preserves the postharvest quality of cut roses through enhancing the antioxidant system. Plants, 11(20): 2713.
  40. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7(9): 405-410.
  41. Mubarok, S.; Suminar, E.; Abidat, A.; Setyawati, C.; Setiawan, E.; Buswar, A. 2023. Overview of Melatonin's Impact on Postharvest Physiology and Quality of Fruits. ActaHorticulturae, 1366: 585-592.
  42. Pang, L.; Wu, Y.; Pan, Y.; Ban, Z.; Li, L.; Li, X. 2020. Insights into exogenous melatonin associated with phenylalanine metabolism in postharvest strawberry. Postharvest Biology and Technology, 168: 111266.
  43. Rashidiani, N.; Nazari, F.; Javadi, T.; Samadi, S. 2020. Comparative postharvest responses carnation and chrysanthemum to synthesized silver nanoparticles (Agnps). Advances in Horticultural Science, 34(2): 133-145.
  44. Reiter, R.J.; Tan, D.X.; Zhou, Z.; Cruz, M.H.; Fuentes-Broto, L.; Galano, A. 2015. Phytomelatonin: Assisting plants to survive and thrive. Molecules, 20(4): 7396-7437.
  45. Wang, P., Sun, X., Chang, C., Feng, F., Liang, D., Cheng, L. and Ma, F. 2013. Delay in leaf senescence of Malushupehensis by long-term melatonin application is associated with its regulation of metabolic status and protein degradation. Journal of Pineal Research, 55(4): 424-434.
  46. Wang, Y.; Guo, M.; Zhang, W.; Gao, Y.; Ma, X.; Cheng, S.; Chen, G. 2023. Exogenous melatonin activates the antioxidant system and maintains postharvest organoleptic quality in Hami melon (Cucumismelo var. inodorus Jacq.). Frontiers in Plant Science, 14: 1274939.
  47. Wang, D., Chen, Q., Chen, W., Guo, Q., Xia, Y., Wu, D., Jing, D., & Liang, G. (2021). Melatonin treatment maintains quality and delays lignification in loquat fruit during cold storage. Scientia Horticulturae, 284, 110126.
  48. Wang, Y., Zhang, J., Ma, Q., Zhang, X., Luo, X., & Deng, Q. (2022). Exogenous melatonin treatment on post-harvest jujube fruits maintains physicochemical qualities during extended cold storage. PeerJ, 10, e14155.
  49. Wu, C., Hao, W., Yan, L., Zhang, H., Zhang, J., Liu, C., & Zheng, L. (2023). Postharvest melatonin treatment enhanced antioxidant activity and promoted GABA biosynthesis in yellow-flesh peach. Food Chemistry, 419, 136088.
  50. Xie, J., Qin, Z., Pan, J., Li, J., Li, X., Khoo, H., & Dong, X. (2022). Melatonin treatment improves postharvest quality and regulates reactive oxygen species metabolism in ‘Feizixiao’ litchi based on principal component analysis. Frontiers in Plant Science, 13, 965345.
  51. Zhao, D.; Wang, R.; Meng, J.; Zhiyuan, L.; Wu, Y.; Tao, J. 2017. Ameliorative effects of melatonin on dark-induced leaf senescence in gardenia (Gardenia jasminoides Ellis): Leaf morphology, anatomy, physiology and transcriptome. Scientific Reports, 7: 1423.
  52. Zhou, C., Luo, L., Miao, P., Dong, Q., Cheng, H., Wang, Y., Li, D. and Pan, C. 2023. A novel perspective to investigate how nanoselenium and melatonin lengthen the cut carnation vase shelf. Plant Physiology and Biochemistry, 196: 982
Greenhouse Plant Production Journal
Volume 1, Issue 4
Autumn 2024
Pages 93-112

  • Receive Date 01 December 2024
  • Revise Date 17 December 2024
  • Accept Date 17 December 2024
  • First Publish Date 17 December 2024
  • Publish Date 01 December 2024

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